Laccase Immobilization on Copper-Magnetic Nanoparticles for Efficient Bisphenol Degradation.
Journal of microbiology and biotechnology(2023)
摘要
Laccase activity is influenced by copper (Cu) as an inducer. In this study, laccase was immobilized on Cu and Cu-magnetic (Cu/FeO) nanoparticles (NPs) to improve enzyme stability and potential applications. The Cu/FeO NPs functionally activated by 3-aminopropyltriethoxysilane and glutaraldehyde exhibited an immobilization yield and relative activity (RA) of 93.1 and 140%, respectively. Under optimized conditions, Cu/FeO NPs showed high loading of laccase up to 285 mg/g of support and maximum RA of 140% at a pH 5.0 after 24 h of incubation (4°C). Immobilized laccase, as Cu/FeO-laccase, had a higher optimum pH (4.0) and temperature (45°C) than those of a free enzyme. The pH and temperature profiles were significantly improved through immobilization. Cu/FeO-laccase exhibited 25-fold higher thermal stability at 65°C and retained residual activity of 91.8% after 10 cycles of reuse. The degradation of bisphenols was 3.9-fold higher with Cu/FeO-laccase than that with the free enzyme. To the best of our knowledge, laccase immobilization on Cu or Cu/FeO NPs has not yet been reported. This investigation revealed that laccase immobilization on Cu/FeO NPs is desirable for efficient enzyme loading and high relative activity, with remarkable bisphenol A degradation potential.
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关键词
Bisphenol A,copper-magnetic nanoparticle,covalent immobilization,laccase,reusability
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