Low CD49d Expression in Newly Diagnosed Chronic Lymphocytic Leukaemia May be Associated with High‐Risk Features and Reduced Treatment‐Free‐Intervals .

This study was carried out to assess the prognostic power of low CD49d expression (≥10%) in newly diagnosed CLL patients using a previously described cohort. 85 patients were included. Median age at diagnosis; 70 years (43-88); CD49d was expressed in 33/85 (38.8%); 23/33 (69.7%) at ≥30% referred to as 'HiCD49d' and 10/33 (30.3%) between 10 and 30% with a bimodal pattern on scatterplot analysis referred to as 'LoCD49d'. Eleven patients (12.9%) presented as Binet stage B, of whom 8 (72.7%) were CD49d+ve (HiCD49d 7/8; LoCD49d 1/8). Seven of 81 patients (8.2%) were NOTCH1 mutated and all were CD49d+ve (p =<0.01). IgVH analysis was performed on 29 (87.8%) of the CD49d+ve cases, of whom 21 (72.4%) were unmutated and 8 (27.6%) were mutated. CD38+ve/CD49d+ve accounted for 11/20 (55%) (CD38+ve/HiCD49D: 9/11; CD38+ve/LoCD49D: 2/11). At 42 months, treatment had been initiated in 18/85 (21%) patients, of these 10/33 (30.3%) were CD49d+ve versus 8/52 (15.4%) of the CD49d-ve group. The median treatment free interval for the CD49d+ve group was 11 months (HiCD49d; 14.5 months, LoCD49d; 11 months) compared to 21.5 months for the CD49d-ve group. These findings suggest that the predictive value of CD49d expression is retained at expression levels down to 10%.