Dataset for Proteomic Analysis of Arylamine N-Acetyltransferase 1 Knockout MDA-MB-231 Breast Cancer Cells

Arylamine N-acetyltransferase 1 ( NAT] ) is frequently upreg-ulated in breast cancer. An unbiased analysis of proteomes of parental MDA-MB-231 breast cancer cells and two sepa-rate NAT] knockout (KO) cell lines were performed. Among 4,890 proteins identified, 737 and 651 proteins were found significantly ( p < 0.01) upregulated and downregulated, re-spectively, in NAT] KO cells, compared to the parental cells. Each set of proteins was analyzed to identify Gene On-tology biological processes, molecular functions, and cellu-lar components that were enriched in the set. Among the proteins upregulated in NAT] KO cells, processes associated with MHC major histocompatibility complex I-mediated anti-gen presentation were significantly enriched. Multiple pro-cesses involved in mitochondrial functions were collectively downregulated in NAT] KO cells, including multiple sub-units of mitochondrial ATP synthase (Complex V of the elec-tron transport chain). This was accompanied by a reduc-tion in cell cycle-associated proteins and an increase in pro-apoptotic pathways in NAT] KO cells. The current dataset