Biometric measurements and genetic instability assessment of in vitro micro-shoots culture of Populus alba

Populus alba is a large essential woody deciduous tree producing the finest wood types. This work’s design depends on in vitro work on Murashige and Skoog basal medium (MS medium) for shooting then rooting multiplication; with change in two factors concentrations (solutes’ concentration “media power” and sucrose concentrations) resulted in 12 treatments. The results showed that there were six responses measured in the new generated individuals: plantlet fresh weight, shoot length, root length, leaf length, leaf number, and roots number. The outcomes illustrated some morphological parameters had a significant difference, like shoot length, root and leaf numbers. While all the physiological measurements had significant differences. The molecular marker random amplified polymorphic DNA (RAPD-PCR) was used to estimate the variation in the new individuals from treatments with the two factors. Four RAPD primers were applied and gave reproducible bands with a total polymorphism percentage of 38.33%. This work’s main issue was to prove that any change in the in vitro growth medium (ex., different solute concentration “media power” and sucrose concentrations) leads to differences in morphology, physiology, and molecular responses. Previously, it was known that new individuals resulted from the tissue culture technique were identical to the mother plant and each other. So, this study clarified the conflict of this issue.