Highly Fluorinated Peptide Probes with Enhanced In Vivo Stability for F-19-MRI

A labeling strategy for in vivo F-19-MRI (magnetic resonance imaging) based on highly fluorinated, short hydrophilic peptide probes, is developed. As dual-purpose probes, they are functionalized further by a fluorophore and an alkyne moiety for bioconjugation. High fluorination is achieved by three perfluoro-tert-butyl groups, introduced into asparagine analogues by chemically stable amide bond linkages. d-amino acids and beta-alanine in the sequences endow the peptide probes with low cytotoxicity and high serum stability. This design also yielded unstructured peptides, rendering all 27 F-19 substitutions chemically equivalent, giving rise to a single F-19-NMR resonance with <10 Hz linewidth. The resulting performance in F-19-MRI is demonstrated for six different peptide probes. Using fluorescence microscopy, these probes are found to exhibit high stability and long circulation times in living zebrafish embryos. Furthermore, the probes can be conjugated to bovine serum albumin with only amoderate increase in F-19-NMR linewidth to approximate to 30 Hz. Overall, these peptide probes are hence suitable for in vivo F-19-MRI applications.