Abstract 2694: An anatomic proteomic atlas of human glioblastoma

Abstract Glioblastoma (GBM) is an aggressive brain tumor with an expected survival of under 15 months despite spirited multimodal therapy. This grim outlook has remained virtually unchanged over the last 40 years and thus necessitates alternative research and therapeutic development strategies. Historically, traditional models of cancer biology have largely considered GBM tissue to be a homogenous mass. Emerging studies however now suggests that the interaction of tumor cells with various normal components of the brain and immune system play an important role in helping cancer grow and develop resistance to therapies. Better understanding of these hallmark features of GBM, collectively known as the “tumor microenvironment” (TME), could lead to the development of new and more effective therapies. In light of this, there is a renewed interest in revisiting our theories of cancer and preserving the microscopic anatomy of GBM in our molecular profiling efforts. Here we leverage laser capture microdissection (LCM) and mass spectrometry-based proteomics, in order to generate a detailed map of the distribution of proteins within tissues across a large number of patients. Specifically, we will isolate, and profile well-understood microscopic components of GBM: cellular tumor (CT), microvascular proliferation (MVP), infiltrating tumor (IT), palisading cells around necrosis (PAN) and normal brain tissue (LE). All of this data will be provided in an online-based GBM atlas as a publicly available resource. This atlas and the associated database for clinical and genomic data will serve as a useful platform for developing therapeutics and testing novel hypotheses related to GBM biology. Citation Format: K.H. Brian Lam, Ugljesa Djuric, Ihor Batruch, Maxime Richer, Phedias Diamandis. An anatomic proteomic atlas of human glioblastoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2694.