Characterization on structure and bioactivities of an exopolysaccharide from Lactobacillus curvatus SJTUF 62116
International Journal of Biological Macromolecules(2022)
摘要
This study aimed to investigate the chemical structure, physicochemical properties, antioxidant capacity, antibacterial ability and anti-biofilm formation activity of an exopolysaccharide (EPS) produced by Lactobacillus curvatus SJTUF 62116 from the fish Gymnocypris przewalskii. The purified EPS, denoted as EPS-1, was mainly composed of glucose and mannose at a relative molar ratio of 1:1.05 with molecular weight of 31.9 kDa. The chemical structure of EPS-1 was consisted of →2)-α-D-Manp-(1→, →4)-α-D-Manp-(1→, →3,6)-α-D-Manp-(1→, T-β-D-Glcp-(1→, →6)-β-D-Glcp-(1→, and →3)-β-D-Glcp-(1→ glycosidic bonds. A sheet-like structure of dried EPS-1 was determined by scanning electron microscope (SEM), whilst a peak-shaped structure of EPS-1 was observed by atomic force microscope (AFM). The degradation temperature of EPS-1 was determined as 300.21 °C using thermogravimetric analysis (TGA). Moreover, the antioxidant capacity of EPS-1 at a concentration of 5.0 mg/mL against DPPH and ABTS was 84.50% and 92.53%, respectively. Furthermore, EPS-1 exhibited acceptable bacteriostatic efficacy against S. Enteritidis, E. coli, and S.aureus with significant inhibition of S. Enteritidis biofilm formation.
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关键词
Exopolysaccharide,Lactobacillus curvatus,Antioxidation activity,Antibacterial activity,Anti-biofilm formation activity
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