Marinobacterium arenosum sp. nov., isolated from a coastal sand

A Gram-negative, aerobic, motile, rod-shaped bacterium, designated strain CAU 1594 T , was isolated from a coastal sand sample collected in the Republic of Korea. Cells of strain CAU 1594 T grew best at 30 °C, pH 7.5, and in the presence of 1% (w/v) NaCl. Phylogenetic analysis, based on 16S rRNA gene sequencing, indicated that strain CAU 1594 T was affiliated with the genus Marinobacterium and most similar to Marinobacterium jannaschii ATCC 27135 T (95.1%) and Marinobacterium stanieri ATCC 27130 T (94.9%). The whole genome of strain CAU 1594 T was 4,917,683 bp, including 4,188 CDSs, with a 60.4 mol% G + C content. Based on draft genome sequences, the average nucleotide identity and digital DNA–DNA hybridization values of strain CAU 1594 T were within the ranges of 71.9−73.1% and 20.0–2.1%, respectively, compared to reference strains. The major respiratory quinone was ubiquinone-8, and the major fatty acids were C 16:0 , summed feature 3 (C 16:1 ω 6 c and C 16:1 ω 7 c ), and summed feature 8 (C 18:1 ω 6 c and C 18:1 ω 7 c ). The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. Based on these results, strain CAU 1594 T represents a novel species of the genus Marinobacterium , for which the name Marinobacterium arenosum sp. nov. is proposed. The type strain is CAU 1594 T (=KCTC 82405 T =MCCC 1K05672 T ).