Composition and contamination of source separated food waste from different sources and regulatory environments

Food waste recycling is needed to create a more sustainable, circular food system; however, the process must be carefully managed to avoid the introduction and build-up of contaminants. We collected and screened source-separated food waste for five classes of contaminants (physical contaminants, heavy metals, halogenated organics, pathogens and antibiotic resistance genes) from two regulatory environments (voluntary vs mandated food separation) to quantify contamination. Physical contamination was frequently found; 57% of samples contained non-compostable waste. Most heavy metals were not detected, and although copper and zinc were present in most samples, they were always below the most stringent global standards for compost. Some samples had detectable halogenated organics, including perfluoroalkyl substances (PFAS), which is cause for concern because some of these accumulate in the food chain. PFBA was detected in 60%, PFHxS in 8% and PFNA in 4% of samples tested. The pathogen Salmonella was present in 3% (2/71) and L. monocytogenes in 11% (8/71) of samples. Shiga toxin-producing E. coli was not detected. Next generation sequencing showed the presence of several genera that contain foodborne pathogens, most commonly Yersinia. Antibiotic resistance genes tet(M) and blaTEM were present in 96% and 97% of samples respectively, however the last-resort colistin resistance gene mcr-1 was not detected. Overall contamination in our source-separated samples was low, with the exception of some antibiotic resistance genes, however our processing method might have underestimated packaging-associated contamination. Regulatory environment did not affect contamination, but carbon, nitrogen phosphorus, calcium, copper, tet (M), and physical contamination varied by source type.