The m6A RNA methylation regulators related transcriptome for identification of pancreatic cancer subtypes and prognostic markers

N6-methyladenosine (m6A) methylation is a major epigenetic modification of RNA that affects processes such as translation of related mRNAs and non-coding RNAs. A large number of recent studies have shown that m6A modifications play a crucial role in cancer development, however, the prognostic value of the m6A associated transcriptome in pancreatic cancer has rarely been investigated. The purpose of this study is to investigate the prognostic markers and prognostic subtypes of m6A RNA methylation regulators related transcriptome in pancreatic ductal adenocarcinoma (PDAC). First, we identified the m6A RNA methylation regulators related prognostic transcriptome by Pearson correlation analysis and univariate cox regression. Subsequently, to explore key prognostic markers from the prognostic transcriptome, we proposed a G-P model based on greedy algorithms and pruning algorithms to obtain a set of key genes CASC11, KRT14, PDZD4, and identified two high/low-risk subtypes of PDAC with significant prognostic differences based on key genes. The clustering Silhouette coefficients was 0.99 for the key genes. In addition, CASC11 and KRT14 were strongly upregulated in the high-risk subtype and PDZD4 was upregulated in the low-risk subtype, and their differential expression was significantly associated with survival. In conclusion, we revealed the typing role and prognostic value of the m6A RNA methylation regulators-associated transcriptome in PDAC and provided new insights for identifying predictive biomarkers and therapeutic targets for PDAC.