Corrigendum: A Systematic Study of the Stability, Safety, and Efficacy of the de novo Designed Antimicrobial Peptide PepD2 and Its Modified Derivatives Against Acinetobacter baumannii

Antimicrobial Activity is Related to the Lipid Composition of PathogensUnlike polymyxins, our peptide could kill Gram(+) bacteria such as Staphylococcus aureus and Staphylococcus epidermidis (Supplementary Figure 2), suggesting that our peptides do not function via LPS binding. When testing other Gram(+) bacteria, we noticed that our peptides were not effective against Enterococcus faecalis at the concentrations used (32 μg/mL and lower). To examine whether the discrimination comes from the bacterial membrane differences, the membrane lipids of these two bacteria were extracted by methanol and chloroform, and TLC was used to analyze the lipid composition (Supplementary Figure 3). The data showed that E. faecalis had much lower contents of phosphatidylethanolamine (PE) and phosphatidylserine (PS) than A. baumannii (Figure 5)