Systematic prompting the new physiological and morphological, biological responses of modified metabolic induction process of Calendula officinalis L under heat stress with couple of supplementary nutritional applications as safety of floriculture crops

Abstract Purpose the objective of study to modified the metabolism of Calendula officinalis L. under heat stress couple with N and P application in two years of study. Methods the trail was conducted during peak summer season (months of May, June, and July) couple with different levels of N & P application to monitor the quality and quantity characterization of marigold (Calendula officinalis L.). The traits of three doses of N and P (0.6 g, 0.9 g, and 0.8 g, 1− 1 g) given to Calendula officinalis L.) to display the vegetative, reproductive, physiological parameters such as Malondialdehyde (MDA), Chlorophyll contents, Lipid Peroxidation Assay in leaves, Li and K contents, phytochemicals and nitrogen and phosphorus used efficacy Results showed that maximum values of Malondialdehyde (MDA), Chlorophylls, Lipid Peroxidation Assay in leaves observed followed by the alteration in Li and K measured in the month of May, June with slight differences July. The phytochemicals like total phenolic contents (84.41 mg GAE/g), total antioxidants (36.3% DPPH), total carotenoids and total flavonoids contents (16.2 and 0.9 mg/100 g) were measured by 0.9 g of Nitrogen application and followed by 1 g of Phosphorus in both years of study. The respirational changes were observed in the higher rate of P levels. The liner changes of N and P rates showed some fluctuation in heat stress months. Conclusions It was concluded that the higher doses of both N and P fertilizers were effective in controlling the heat stress and mentioned the quality of florets by various induction and biological active process.