A Novel HPTLC Method for Simultaneous Quantification of (-)-Pseudosemiglabrin and Isoglabratephrin a Bioactive Prenylated Flavonoids Identified in Tephrosia Apollinea

Fingerprint analysis by HPTLC is an effective and powerful tool for linking the bioactive components profile of the plants with botanical identity and for estimation of chemical biomarkers. Tephrosia apollinea has been used in folk medicine to treat inflammation and relieving pain, as well as for diarrhea and abdominal pain. A densitometric High Performance Thin Layer Chromatography (HPTLC) fingerprinting method has been developed for the simultaneous determination and quantification of (-)-pseudosemiglabrin (SSG) and isoglabratephrin (IGP) in different parts of Tephrosia apollinea collected in summer and winter. The study revealed that SSG and IGP are the major components in T. apollinea and found in all parts of the plant at different amount. The proposed HPTLC method was found to be convenient for rapid screening of active components present in ethanol extracts of T. apollinea and can be used for analysis and routine quality control of the raw materials as well as formulations containing Tephrosia species.