Ameliorative effect of marine macro-algae against carbon tetrachloride (CCl4) induced hepatic fibrosis and associated complications in rats

Objectives: Liver fibrosis is one of the serious health concerns around the globe. Persistent exposure to drugs, toxicants and pathogens may induce liver fibrosis. Marine macro algae have been globally consumed due to nutritive and medicinal value. This study was conducted to evaluate the protective role of two seaweeds Padina pavonia and Caulerpa racemosa in CCl4-induced liver fibrosis in rats. Materials and methods: animal model of hepatic fibrosis was developed by injecting 40% carbon tetrachloride (CCl4) dissolved in olive oil (2ml/kg, b.w., i.p.) on alternate days for 30 days. Water extracts [(200mg/kg body weight (b.w.) p.o.] of P. pavonia and C. racemosa were given to rats daily for 30 days. On day 31, rats were sacrificed after 12 h fasting. Serum was used for biochemical estimation. 10% neutral buffered formalin was used to preserve the liver sample for histopathological examination, while the other portion was used for the preparation of tissue homogenate to estimate antioxidant enzymes and malondialdehyde levels.