Analysis of Hepatitis C Virus Superinfection Exclusion by Using Novel Fluorochrome Gene-Tagged Viral Genomes

Volume 81, no. 9, p. 4591–4603, 2007. Page 4593, Materials and Methods, Plasmid construction, lines 4 to 16: We have discovered that there is an error in the description of the plasmid construction of fluorochrome gene-tagged reporter plasmids. We performed two separate PCRs, using antisense primer Alinker_Xba_Pme_NS5A_aa383 (5 -TTAAACCCAG GTCTAGAACCGCTCGAGGGGGGCTGGCCAAAG-3 ) and sense primer S6660 (5 -CAGGACTGACCACTGACAATC TG-3 ) or sense primer Slinker_Xba_Pme_NS5A_aa383 (5 -TCTAGACCTGGGTTTAAACGTGATGCAGGCTCGTCCA CGGG-3 ) and antisense primer A7759 (5 -CAGACTCCAGGTCCGGATCTCCAGGC-3 ) for insertion of the linker at NS5A amino acid position 383 and antisense primer Alinker_Xba_Pme_NS5A_aa378 (5 -TTAAACCCAGGTCTAGACTG GCCAAAGGTCTTGATGGCCAG-3 ) and sense primer S6660 or sense primer Slinker_Xba_Pme_NS5A_aa378 (5 -TCT AGACCTGGGTTTAAACGTCCCCCCTCGAGCGGTGATGCAG-3 ) and antisense primer A7759 for insertion at NS5A amino acid position 378, respectively. Amplicons were combined by a second PCR resulting in fragments that comprise the complete NS5A coding region plus linker, which were SanDI/RsrII digested and transferred into pFKi389LucNS33 _dg_JFH. We apologize for any inconvenience this may have caused to other investigators.