Pseudaminobacter soli sp. nov., Isolated from Paddy Soil Contaminated with Heavy Metals

A Gram stain-negative, aerobic, rod-shaped strain, designated HC19 T , was isolated from heavy metals contaminated paddy soil. The 16S rRNA gene-based phylogenetic analysis indicated that strain HC19 T belonged to the genus Pseudaminobacter , and shared 97.0% 16S rRNA gene sequence similarity with P. manganicus JH-7 T , and less than 97% similarities with other type strains belonging to the genus. The major cellular fatty acids were C 19:0 cyclo ω 8 c (55.0%) and C 18: 1 ω 7 c (18.7%). The major quinone was ubiquinone Q-10. The major polar lipids were phosphatidylmonomethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of HC19 T and P. manganicus JH-7 T were 68.0% and 22%, respectively. The G+C content of the genomic DNA was 63.3 mol%. On the basis of phenotypic, chemotaxonomic, and genotypic data, strain HC19 T is considered as a novel species in the genus Pseudaminobacter , for which the name Pseudaminobacter. soli sp. nov. is proposed. The type strain is HC19 T (= KCTC 82870 T = CCTCC AB 2021107 T ).