A study on preserving endometrial glandular architecture during preparation using BD SurePath (TM) liquid-based cytology reagents: Cellular fixation with preservative fluid requires at least 18 h
CYTOPATHOLOGY(2022)
摘要
Introduction This study aimed to determine the causes of disruption of the three-dimensional architecture of endometrial glands prepared using BD SurePath (TM) liquid-based cytology (SP-LBC) reagents. One sample preparation method for endometrial cytology is presented in which this three-dimensional architecture can be retained. Methods SP-LBC specimens were prepared by the following three methods: (1) using the BD PrepMate(TM) (PrepMate) System after cellular fixation for 1-6 h (method A); (2) without using the PrepMate System after cellular fixation for 1-6 h (method B); and (3) using the PrepMate System after cellular fixation for at least 18 h (method C). Size and numbers of endometrial cell clusters and numbers of solitary scattered cells were then evaluated. Results Significantly higher numbers of cell clusters with a major axis of 200 mu m or more were yielded by method C (71.3 +/- 57.2) than methods A (9.3 +/- 5.9, P < 0.001) or B (44.3 +/- 28.8, P < 0.05). Method B yielded significantly higher numbers of cell clusters than method A (P < 0.001). Method A (132.2 +/- 107.7, p < 0.001) yielded significantly higher numbers of solitary scattered cells than methods B (29.1 +/- 14.8) and C (35.7 +/- 23.3). No significant difference in solitary cell numbers was found between methods B and C. Conclusions Retention of endometrial glandular architecture is rendered possible by allowing sample fixation times of 18 h or more when preparing specimens using the PrepMate System.
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关键词
cellular fixation, endometrial cytology, endometrial glandular architecture, liquid based cytology, preservative fluid
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