Documenting More Linezolid Resistant Staphylococcus Aureus (Lrsa) Strains Collected From Orthopedic Infections In Pakistani Population: A Prospective Study Of Anti-Microbial Resistance (Amr)

Orthopedic infection is one of the most common surgical complications which involves emotional as well as physical trauma. Furthermore, microbial resistance (MDR) may uplift the risks associated with morbidity, mortality, duration and ultimate cost of treatment. The current study was aimed to determine the pathogenic microbes and their sensitivity pattern isolated from pus samples. The prospective cross-sectional study was conducted at Ghurki Trust Teaching Hospital in Lahore (Pakistan) to access antimicrobial susceptibility pattern of bacterial isolates from orthopedic post-surgical wound infections and their sensitivity to commonly used antibiotics among patients who were presented with wound infection related to any type of orthopedic surgery during January 2019- January 2020. Samples were collected from the wound infection through culture swab tube and aspirate by disposable syringes. The susceptibility pattern of studied bacterial pathogens was determined by Kirby-Bauer disc diffusion technique. Of 518 bacterial isolates, the most common pathogen was methicillin-sensitive Staphylococcus aureus (MSSA) (29.8%) and Escherichia coli (16.2%). All of the Gram-positive microorganisms showed high level of resistance to penicillin, ampicillin and co-trimoxazole. Vancomycin showed maximum sensitivity to all Gram-positive microbes especially in methicillin-resistant Staphylococcus aureus (MRSA). However, the linezolid showed less sensitivity than vancomycin in MRSA. Gram-negative microbes showed multi-drug resistance and carbapenems and piperacillin-tazobactam resistant strains were also observed in Acinetobacter baumannii and Pseudomonas aeruginosa. Most of the Gram-positive isolates were resistant to penicillins and Gram-negative isolates to almost all their first line agents. Less sensitivity of linezolid (LRSA) was reported as compared to vancomycin. For the prevention of AMR, it is necessary to perform the antibiotic susceptibility test for the better management of wound infections and selection of most appropriate treatment on rational basis.