Human Acetyl-Coa Acetyltransferase-2 (Acat-2) Gene Was Associated With Hyperlipidemia In Han Chinese Population: A Case-Control Study

Background: ACAT2 is a ubiquitous class of enzymes involved in biosynthesis pathway of cholesterol. Hyperlipidemia is a strong predictor for coronary artery disease (CAD). Little is known about the association of ACAT2 gene with hyperlipidemia. The aim of the present study was to assess the relationship between the human ACAT2 gene and hyperlipidemia by conducting case-control study. Methods: A total of 233 hyperlipidemia patients and 526 controls were genotyped for the three SNPs used as genetic markers for the human ACAT2 gene (rs3798211, rs15892, and rs3465). Three SNPs were selected from the HapMap website and genotyped by using TaqMan (Applied Biosystems) Genotyping Assays. Data analysis was performed by using SPSS 17.0 and haploview 4.0. Results: The distributions of the genotypes and alleles in three SNPs (rs3798211, rs15892, and rs3465) were significantly different between the hyperlipidemia patients and the control subjects (all P=0.000, respectively). We have found that the frequencies of all three SNPs were significantly higher in hyperlipidmia patients when compared to the controls in a dominant model and the significance remained after adjusting multivariable confounders, such as sex, age, BMI, SBP, DBP, glucose, smoking, drinking, education and physical activity by using multiple logistic regressions (Odds ratio=1.336, 95% CI: 1.188-1.502; Odds ratio=1.410, 95% CI: 1.034-1.924; Odds ratio=2.754, 95% CI: 1.610-4.114; all P=0.000, respectively). Further, the triglycerides, total cholesterol, and low density lipoprotein-cholesterol concentrations were significantly higher in the TT genotype of rs3798211, and GG genotype of rs3465 when compared to other genotypes of the two SNPs after adjusting age, sex, smoking, drinking, education and physical activity in hyperlipidemia patients and control subjects (all P<0.05). Conclusions: The results of this study indicate that TT genotype of rs3798211, AA genotype of rs15892, and GG genotype of rs3465 in human ACAT2 gene might be the possible risk genetic marker for hyperlipidemia in Han Chinese population.