Screening Of Streptomycetes-Cellulolytics For Processing Crop Production Waste

In the composition of plant residues, the most common carbohydrate polymer is cellulose. Cellulose bioconversion is complicated by the complexity of enzymatic hydrolysis processes. Effective utilization of crop waste to obtain new pconoinically valuable products requires effective microorganisms - producers of cellulases. The aim of this work is to identify new strains with cellulase activity for use in technologies for processing plant waste. Streptomycetes were isolated from various natural sources. The ability to metabolize carboxymethylcellulose (endogluconase activity) and microcrystalline cellulose (cellobiohydrolase activity) was determined in natural isolates. Groups of active, moderate and weak potential cellulose destructors are identified. High activity of cellulases among 130 streptomycetes cultures was typical for 15% (20 strains). Active strains of Streptomyces sp. 1.1, Streptomyces sp. K 7.5, Streptomyces sp. 1.10, and Streptornyces sp. LOS 2-8 were detected at a value of carbox ym othylcellulose destruction zones greater than 59 mm in the congo-red test. When determining the activity of cellobiohydrolase (by the number of reduced sugars), the most active strains were S. felleus 3T-12 (398.77 c.u./(g . 10 min)) and S. noboritoensis 1T-14 (258.06 c.u./(g . 10 min)), isolated from soils of subtropical climate. Among rhizospheric isolates from sod-podzolic soil, the strain was the most active S. anulatus T-2-20 (263.55 c.u./(g . 10 min)), which previously showed antifungal and phytoregulatory activity. The studied strains can be used for application in technologies of utilization of plant waste in agriculture.