Hybrid Glucose/O-2 Biofuel Cell Based On As Pyroquinoline Quinone Glucose Dehydrogenase At The Bioanode

Implantable bioelectronics devices need internal power sources that are capable of delivering adequate electrical power. Here we report a hybrid glucose/O-2 biofuel cell wherein the glucose bioanode of pyroquinoline quinone glucose dehydrogenase (PQQ-GDH) modified chitosan buckypaper (BP) is coupled with an oxygen reducing cathode constructed from bacterial nanocellulose (BNC) pellicle crosslinked with multi-walled carbon nanotubes (MWCNTs) coated with gold and colloidal platinum (co-Pt). The flexible and electrically conductive enzyme functionalized BP-PQQ-GDH bioanode and enzyme-free BNC-Au-co-Pt biocathode harnessed the biochemical energy of glucose via the oxidation of glucose and reduction of molecular oxygen to generate electrical power. The hybrid biofuel cell system exhibited an open circuit voltage and power density of 476 mV and 66.56 mu W/cm(2), respectively, with a current density of 654 mu A/cm(2) in the presence of 20 mM glucose. At physiological glucose concentration, the biofuel cell exhibited an open circuit voltage and power density of 453 mV and 45.54 mu W/cm(2), respectively, with a current density of 594 pA/cm 2. This facile strategy to prepare flexible conductive electrodes for the development of hybrid glucose/O-2 biofuel cell system can be readily extended to diverse applications in hybrid glucose biofuel cell and biosensor technology.