Up-Regulation Of Mir-200b By Baicalin To Inhibit Migration Of Mcf-7 Breast Cancer Cells

The incidence of breast cancer is increasing by years with the transition of life styles. The metastasis of cancer is one major challenge. MicroRNA (miR) plays crucial roles in the pathogenesis and progression of tumors. Previous study has shown that miR-200b could inhibit tumor cell migration. Baicalin is one flavonoids compound extracted from scutellaria of family Labiatae. It has multiple pharmaceutical activities including up-regulating miR expression in treating certain diseases. In pilot study, we found Baicalin could inhibit tumor cell migration but did not know its correlation with miR-200b. Breast cancer cell line MCF-7 was firstly tested under MTT assay to observe the effect of Baicalin on cell survival rate, followed by scratch assay targeting cell migration. Real-time qPCR was then used to detect the expression level of miR-200b, along with migrating related protein E-cadherin. Small molecule inhibition of miR was also employed to observe possible mechanisms. Baicalin (0 similar to 40 mu M) had no significant inhibition on MCF-7 cell survival. Drugs at 80 mu M had an inhibitory rate at 75.03%+/- 3.06%. Baicalin can also upregulate miR-200b level and E-cadherin protein expression, thus inhibiting MCF-7 cell migration. MiR-200b might be the target for the inhibition of cell migration by baicalin. This novel mechanism of tumor cell migration inhibition by baicalin provides new insights for developing novel treatment strategy in clinics.