Neural basis of functional ultrasound signals

Functional ultrasound imaging (fUSI) is a popular method for studying brain function, but it remains unclear to what degree its signals reflect neural activity on a trial-by-trial basis. Here, we answer this question with simultaneous fUSI and neural recordings with Neuropixels probes in awake mice. fUSI signals strongly correlated with the slow (<0.3 Hz) fluctuations in firing rate measured in the same location and were closely predicted by convolving the firing rate with a 2.9 s wide linear filter. This filter matched the hemodynamic response function of awake mouse and was invariant across mice, stimulus conditions, and brain regions. fUSI signals matched neural firing also spatially: recordings with two probes revealed that firing rates were as highly correlated across hemispheres as fUSI signals. We conclude that fUSI signals bear a simple linear relationship to neuronal firing and accurately reflect neural activity both in time and in space. ### Competing Interest Statement A.U. is the founder and a shareholder of AUTC, a company commercializing neuroimaging solutions for preclinical and clinical research.