Low-Temperature Water Uptake of Individual Marine and Biologically Relevant Atmospheric Particles Using Micro-Raman Spectroscopy

The interaction of water vapor and the water uptake behavior of atmospheric particles are often investigated as a function of relative humidity (0-100% RH) at ambient temperature. However, lower temperature studies are important to understand how atmospheric particles nucleate ice through various mechanisms including immersion freezing. Immersion freezing requires the formation of a condensed water droplet at lower temperatures prior to freezing. To better understand low-temperature water uptake behavior of marine and biologically relevant atmospheric particles, we have investigated water uptake of single atmospheric particles using a micro-Raman spectrometer coupled to an environmental cell for measurements at lower temperatures and as a function of relative humidity. These particles include sodium chloride, sucrose, Snomax, lipopolysaccharide, and laminarin. Particles range in size from 2 to 3 mu m in diameter and can be monitored by using optical microscopy and Raman spectroscopy as a function of relative humidity at temperatures between 253 and 298 K. From the Raman spectra collected, we can determine a Raman growth factor defined as an increase in the intensity of the O-H stretch as a measure of the integrated water content of a particle compared to the dry particle. These data show that for lipopolysaccharide, laminarin, and Snomax, unlike simple saccharides such as sucrose and other soluble organics, as temperature decreases, water uptake begins at lower relative humidity and does not follow a solubility temperature dependence. This suggests that at lower temperatures the particles are adsorbing water on the surface rather than dissolving and absorbing water. Furthermore, repeated water uptake cycles cause a change in the morphology of some of these particles, which is shown to promote water uptake at lower relative humidity. These results give new insights into water uptake of these different marine and biologically relevant particles at low temperature at subsaturation relative humidity prior to droplet formation and immersion freezing.