Calcineurin A beta gene knockdown inhibits transient outward potassium current ion channel remodeling in hypertrophic ventricular myocyte

It has been shown that the activation of calcineurin is involved in regulating ion channel remodeling in hypertrophic cardiomyocytes. But the precise role of calcineurin in the regulation of transient outward potassium current (I (to)), an ion channel associated with fatal arrhythmia, remains controversial. This study aimed to examine the effects of calcineurin A beta (CnA beta) gene knockdown on I (to) channel remodeling and action potential duration (APD) in the hypertrophic ventricular myocytes of neonatal rats. Results showed that phenylephrine stimulation caused hypertrophy of ventricular myocytes, upregulation of CnA beta protein expression, downregulation of Kv4.2 mRNA and protein expression, a decrease in I (to) current density, and prolongation of APD. CnA beta gene knockdown significantly inhibited the effects of phenylephrine stimulation. Our data indicate that CnA beta gene knockdown can inhibit I (to) channel remodeling and APD prolongation in hypertrophic neonatal rat ventricular myocytes. This finding suggests that calcineurin may be a potential target for the prevention of malignant ventricular arrhythmia in a hypertrophic heart.