Regulation of maternal behavior, social isolation responses, and postpartum estrus by steroid hormones and vaginocervical stimulation in sheep.

Periparturient ewes display several activities in addition to maternal behavior per se. They isolate themselves from the flock and, contrary to other mammals, do not show postpartum estrus. We investigated the possibility of a common hormonal control of maternal behavior, prepartum social isolation responses, and absence of postpartum estrus. We quantified responses to flockmate separation and incidence of sexual receptivity at several reproductive stages in intact ewes (Experiment 1). Responses to social isolation were lowest in preparturient ewes and at pregnancy day 149, intermediate at pregnancy day 147 and highest at day 136 and in non-pregnant ewes (P < 0.05 between the 3 levels). In a second experiment, we quantified the same parameters and maternal behavior in 1) ovariectomized ewes receiving medroxyprogesterone acetate only (ovxMPA); 2) ovariectomized ewes receiving MPA + estradiol benzoate (ovxSHORT); 3) intact ewes receiving a longer MPA + estradiol dipropionate treatment, before and after vaginocervical stimulation (VCS). Before VCS no steroid treatments decreased social isolation responses and maternal behavior was scarce or absent. Following VCS and interaction with lamb, maternal responses in the ovxSHORT group increased while social isolation responses decreased. Sexual receptivity occurred in non-pregnant ewes and in ovxSHORT group. Conclusion: some hormonal treatments +VCS can effectively induce maternal behavior and reduce social isolation responses. Long-term progestin treatment can inhibit postpartum estrus.