Acquisition And Loss Of Ctx-M Plasmids In Shigella Species Associated With Msm Transmission In The Uk
MICROBIAL GENOMICS(2021)
摘要
Shigellosis in men who have sex with men (MSM) is caused by multidrug resistant Shigellae, exhibiting resistance to anti-microbials including azithromycin, ciprofloxacin and more recently the third-generation cephalosporins. We sequenced four bla(CTX-M-27)-positive MSM Shigella isolates (2018-20) using Oxford Nanopore Technologies: three S. sonnei (identified as two MSM clade 2, one MSM clade 5) and one S. flexneri 3a, to explore AMR context. All S. sonnei isolates harboured Tn7/ Int2 chromosomal integrons, whereas S. flexneri 3a contained the Shigella Resistance Locus. All strains harboured IncFII pKSR100-like plasmids (67-83kbp); where present bla(CTX-M-27) was located on these plasmids flanked by IS26 and IS903B. however bla(CTX-M-27) was lost in S. flexneri 3a during storage between Illumina and Nanopore sequencing. IncFII AMR regions were mosaic and likely reorganised by IS26; three of the four plasmids contained azithromycin-resistance genes erm(B) and mph(A) and one harboured the pKSR100 integron. Additionally, all S. sonnei isolates possessed a large IncB/0/K/Z plasmid, two of which carried aph(3')-Ib/aph(6)-Id/sul2 and tet(A). Monitoring the transmission of mobile genetic elements with co-located AMR determinants is necessary to inform empirical treatment guidance and clinical management of MSM-associated shigellosis.
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关键词
antimicrobial resistance, CTX-M, ESBL, MSM, public health, Shigella
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