Shoot potassium content provides a physiological marker to screen cotton genotypes for osmotic and salt tolerance

Drought and salinity are considered two major abiotic stresses that diminish cotton production worldwide. Studying common morphological and physiological responses in cotton cultivars may help plant biologists to develop and apply standard screening criteria for either of these stresses and for their combination. Therefore, this research aimed to assess the suitability of several physiological parameters as diagnostic to report on osmotic and salinity tolerance in six elite cotton genotypes. Data for relative growth rate (RGR), RGR-reduction, potassium (K+) concentrations in roots, xylem sap and shoots, stomatal conductance (g(s)) and net photosynthesis rate (P-n) were assessed. Based on RGR and RGR-reduction, we observed an association between osmotic tolerance and salinity tolerance of cotton genotypes. Furthermore, this study found that tolerant cotton genotypes were better able to maintain high RGR, tissue K+, and gas exchange under both hyperosmotic and saline conditions. Shoot K+ levels showed high negative correlations with both osmotic and salinity stress and emerged as a convenient and suitable parameter to assess cotton tolerance to either stress. Novelty statement Cotton (Gossypium hirsutum) is a leading fiber crop that is cultivated in more than 52 countries. Much of the land where cotton is grown faces co-occurring drought and salinity abiotic stress which negatively impacts cotton yield and fiber quality. In the present study, cotton genotypes were identified with tolerance to both hyperosmolarity and salinity. Furthermore, we show that shoot potassium content is a diagnostic trait that reports on both osmotic and salinity stress and hence a convenient tool for screening cotton germplasm.