Effects of power ultrasound treatment on the shelf life of button mushrooms: Digital image processing and microbial counting can reveal the effects

Edible button mushroom (Agaricus bisporus) is the most common commercial-grade mushroom in the world. The shelf life of button mushrooms is limited to a range between two and four days because of enzymatic browning at medium ambient temperature if it is minimally processed. This study aimed to investigate the effects of power ultrasonics and its interaction with several treatments including H2O2 and O-3 on increasing the storage quality of edible button mushroom by controlling enzymatic browning. A 100 W ultrasonic bath with a frequency between 20 and 35 kHz was used during the experiments. The storage quality was studied by examining the changes in color and microbial content over 12 days. The results obtained from the digital image processing and total microbial counting showed that the ultrasonic treatment for 6 min is an appropriate method for controlling the color preservation of mushrooms to improve their shelf life. The maximum changes in RGB band, HSV band, L*a*b* band, and microbial content of the mushroom samples under the ultrasonic treatment were equal to 7%, 12%, 10%, and 11%, respectively. Furthermore, having the color properties and microbial content of the samples, the artificial neural network (ANN) was capable of predicting their storage period with an MSE of 0.011.