Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. isolated from soil

Two Gram-stain negative, yellow-pigmented, and mesophilic bacteria, designated strains R7 T and R19 T , were isolated from sandy and forest soil, South Korea, respectively. Both strains were non-motile rods showing catalase- and oxidase-positive activities. Both strains were shown to grow at 10–37°C and pH 6.0–9.0, and in the presence of 0–1.5% (w/v) NaCl. Strain R7 T contained iso-C 14:0 , iso-C 15:0 , iso-C 16:0 , and summed feature 9 (comprising C 16:0 10-methyl and/or iso-C 17:1 ω 9 c ), whereas strain R19 T contained iso-C 11:0 3-OH, C 16:1 ω 7 c alcohol, iso-C 11:0 , iso-C 15:0 , iso-C 16:0 , and summed feature 9 (comprising C 16:0 10-methyl and/or iso-C 17:1 ω 9 c ) as major cellular fatty acids (> 5%). Both strains contained ubiquin-one-8 as the sole isoprenoid quinone and phosphatidylglycerol, phosphatidylethanolamine, and an unidentified phospholipid as the major polar lipids. The DNA G + C contents of strains R7 T and R19 T calculated from their genomes were 66.9 mol% and 68.9 mol%, respectively. Strains R7 T and R19 T were most closely related to Lysobacter panacisoli C8-1 T and Lysobacter niabensis GH34-4 T with 98.7% and 97.8% 16S rRNA sequence similarities, respectively. Phylogenetic analyses based on 16S rRNA gene sequences showed that strains R7 T and R19 T formed distinct phylogenetic lineages within the genus Lysobacter. Based on phenotypic, chemotaxonomic, and molecular features, strains R7 T and R19 T represent novel species of the genus Lysobacter , for which the names Lysobacter arenosi sp. nov. and Lysobacter solisilvae sp. nov. are proposed. The type strains of L. arenosi and L. solisilvae are R7 T (= KACC 21663 T = JCM 34257 T ) and R19 T (= KACC 21767 T = JCM 34258 T ), respectively.