De novo transcriptome sequencing and comparative profiling of the ovary in partially engorged and fully engorged Haemaphysalis flava ticks.

Haemaphysalis flava is the vector of several pathogens and has medical and veterinary importance. Transcriptome information of the ovary of H. flava is unavailable and limits understanding of its molecular basis of reproduction. We studied the ovary transcriptome of partially engorged and fully engorged H. flava using high-throughput RNA sequencing technology. A total of 53,025,360 and 57,942,890 clean reads were obtained with 7.95 GB and 8.69 GB clean bases in partially engorged ticks (PETs) and fully engorged ticks (FETs), respectively. The clean reads were assembled into 138,711 unigenes. A total of 72,043 unigenes (51.93%) were annotated and 66,668 unigenes (48.07%) were unknown. A total of 38,487 differentially expressed genes (DEGs) were found between PET and FET with 19,031 upregulated genes and 19,456 downregulated genes. The RNA-seq results were validated by qRT-PCR, including six upregulated genes and three downregulated genes. Some unigenes coding for nutrient transporters, proteases, and protease inhibitors were found and analyzed. This study was the first time to perform the transcriptome sequences of the ovary of partially engorged and fully engorged H. flava. The results can benefit the understanding of the molecular basis of ovary maturation and oogenesis of the H. flava and boost the development of the strategies for control of H. flava.