Development and validation of a simple, selective, and sensitive LC-MS/MS assay for the quantification of remdesivir in human plasma

•A fully validated HPLC-MS/MS assay for quantifying remdesivir in human plasma.•Rapid one-step solid-phase extraction was applied using an Ostro plate.•Accuracy (102-105%), precision (<9.8 CV%), and recovery (77%) met FDA Guidelines.•Lower limit of quantitation was 0.5 ng/mL.