Current State Of Development Of Biosensors And Their Application In Foodborne Pathogen Detection

Foodborne disease outbreaks continue to be a major public health and food safety concern. Testing products promptly can protect consumers from foodborne diseases by ensuring the safety of food before retail distribution. Fast, sensitive, and accurate detection tools are in great demand. Therefore, various approaches have been explored recently to find a more effective way to incorporate antibodies, oligonucleotides, phages, and mammalian cells as signal transducers and analyte recognition probes on biosensor platforms. The ultimate goal is to achieve high specificity and low detection limits (1 to 100 bacterial cells or piconanogram concentrations of toxins). Advancements in mammalian cell-based and bacteriophage-based sensors have produced sensors that detect low levels of pathogens and differentiate live from dead cells. Combinations of biotechnology platforms have increased the practical utility and application of biosensors for detection of foodborne pathogens. However, further rigorous testing of biosensors with complex food matrices is needed to ensure the utility of these sensors for point-ofcare needs and outbreak investigations.HIGHLIGHTSBiosensors can be used for pathogen detection in various food matrices.Specificity and sensitivity of biosensors rely on biorecognition molecules and signal output.Working principles of different biosensors can be combined for improved sensitivity.Bacteriophage-based sensors are highly specific and becoming more commonly used.Cell-based sensors can determine the virulence of viable and active pathogens and toxins.