Bioaccessibility Of Microamount Metal Elements In Oyster And Cam Samples Using In Vitro Digestion

A bionic method, in vitro digestion, was used for the pretreatment of oyster and clam samples. The oyster and clam samples were digested at 37 degrees C under the action of bionic digestive juice including saliva, gastric juice and intestinal juice. The contents of trace metals in in vitro digestion extracts ( gastric juice extracts and intestinal juice extracts) were determined by atomic absorption spectrometry. The bioaccessibility of trace metals was assessed using the contents of trace metals in bionic extracts. The results showed that the contents of Fe, Cu, Zn and Cd in gastric juice extracts of in vitro digestion on oysters were higher than those in intestinal juice extracts. The contents of Fe and Cu in gastric juice extracts of in vitro digestion on clam were not obviously different with that of in intestinal juice extracts. Otherwise, the contents of Zn and Cd in gastric juice extracts were much higher than those of in intestinal juice extracts. The bioaccessibility of Fe, Cu, Zn and Cd in oysters using in vitro digestion was 60. 2%, 83. 6%, 83. 1% and 76. 8%, respectively. The bioaccessibility of Fe, Cu, Zn and Cd in clams was 46. 3%, 86. 3%, 85. 3% and 87. 7%, respectively. The results will provide a scientific basis for further researches such as bioaccumulation and bioavailability of trace metal in oysters and clams, evaluation of the health risk of Cd from oyster and clam consumption.