Obtaining Glycosaminoglycans From Tilapia ( Oreochromis Niloticus ) Scales And Evaluation Of Its Anticoagulant And Cytotoxic Activities Glycosaminoglycans From Tilapia Scales: Anticoagulant And Cytotoxic Activities

Food Research International(2021)

引用 6|浏览5
暂无评分
摘要
Large amounts of by-products are generated during fish processing. The study aimed to assess whether tilapia scales are a potential source for obtaining glycosaminoglycans, as well as to determine their anticoagulant and cytotoxic/antiproliferative activities, against different tumor lines. The glycosaminoglycans were extracted, purified, and fractionated. The fractions that indicated the presence of uronic acid and sulfated GAGs were characterized by electrophoresis, NMR, and degree of sulfation (DS). The extraction process using the papain enzyme had a yield of 0.86%. Fraction V (FV) revealed the presence of chondroitin sulfate chains CS-A and CS-C, with DS of 0.146. FV demonstrated anticoagulant potential, as it was able to increase aPTT time. FV showed a cytotoxic effect for HTC metabolizing cells at 24, 48, and 72 h. However, it did not show activity for neuroblastoma cells in any of the evaluated times. The results indicate that the tilapia scales are a possible source for obtaining chondroitin sulfate, with potential use as anticoagulant and cytotoxic/antitumor.
更多
查看译文
关键词
Underutilized fish parts, By-products, GAGs, Chondroitin sulfate, CS-A, CS-C, Bioactivities
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要