Bacterial diversity in intestinal mucosa of mice fed with Dendrobium officinale and high-fat diet

This study aimed to explore the effect of Dendrobium officinale (DO) on the diversity of intestinal mucosal flora in high-fat diet mice and provided an experimental basis for the development and research of DO and its series products. Twenty-four mice were randomly assigned to four equal groups of six mice, namely the control (bcm) group, model (bmm) group, Dendrobium officinale (bdm) group, and positive control (bjm) group. Mice in the bdm group were administrated at the dose of 2.37 g·kg −1 ·days −1 , and those in bjm group were given the Lipid-lowering decoction at the concentration of 1.19 g·kg −1 ·days −1 , and sterile water was used as a placebo control twice a day for 40 consecutive days. We measured the dynamic weight changes and intestinal mucosal flora changes in mice. The analysis showed that DO had a regulatory effect on weight change induced by a high-fat diet in mice. DO could also regulate the changes in the diversity of the intestinal mucosa of mice, which was specifically reflected in the changes of Chao 1, ACE, Shannon and Simpson index. The sample information of the bdm group was relatively concentrated, but the distance from the bmm group was relatively scattered. The relative abundance results showed dominant bacteria phylum (such as Bacteroidetes, Actinobacteria, Verrucomicrobia) and bacterial genus (such as Bifidobacterium, Ruminococcus , Ochrobactrum ) in the intestinal mucosa of the four groups. And significant differences in the major microbiota between the bdm and bjm groups. In addition, DO changed the carbohydrate, energy, and amino acid metabolism of intestinal mucosal flora. To sum up, DO has a regulatory effect on weight change induced by high-fat diet in mice and can improve the diversity of intestinal mucosal flora, promote the abundance of Ochrobactrum , inhibit the abundance of Bifidobacterium and Ruminococcus , and influence the intestinal flora to positively affect high-fat diet-induced negative effects in mice.