Lactoferrin Suppresses Lps-Induced Expression Of Hmgb1, Microrna 155, 146, And Tlr4/Myd88/Nf-Kappa B Pathway In Raw264.7 Cells

Objective: This current study evaluated the underlying mechanisms of LF against the inflammatory microRNAs (miRNAs), HMGB1 expression, and TLR4-MyD88-NF-kappa B pathway in LPS-activated murine RAW264.7 cells.Methods: MTT assay was used to assess cell metabolism and the cell culture levels of the cytokines (TNF-alpha, IL-6) were evaluated by Enzyme-linked immunosorbent assay (ELISA). The expression of miRNAs was quantified by using qPCR and the expression of HMGB1, TLR4, MyD88, and phosphorylated NF-kappa B (P-p65) were determined with Western blot and qPCR, respectively.Results: The results indicated that LF downregulates IL-6 and TNF-alpha expression. LF exhibited the degradation of P-p65 and reduced the production of HMGB1, TLR4, and MyD88 in LPS-induced inflammatory response. Importantly, in parallel with the suppression of cytokines and HMGB1-TLR4-MyD88-NF-kappa B pathway, LF could induce a decrease in inflammatory selected miRNAs, mmu-mir-155, and mmu-mir-146a expression.Conclusions: Altogether, these findings provide LF as a prominent anti-inflammatory agent that could modulate HMGB1, mmu-mir-155, mmu-mir-146a, and TLR4/MyD88/NF-kappa B pathway.