Predicting NTPase Activity For Protein Belonging To E. Coli

The complete human genome has many genes of which few genes are associated with no known function but are expressed in normal as well as disease states. These proteins of unknown function have been reported in database and few structures of these sequences are also deposited in Protein Data Bank. Therefore performing computer-aided in silico analysis would enable to predict possible role using bioinformatics tools and software's. An example of such protein structure deposited in PDB, (id: 1u5w) was selected to assign probable role in biological process. The protein sequence in fasta format was subjected to BLAST search against various protein sequence and structure databases. Analysis resulted in several similarities with other proteins/enzymes, of which Nucleoside triphosphatases were observed with reasonable similarity with the queried protein.