Abstract P183: Human Blood Pressure On Low- Or High-sodium Intake Is Associated With Divergent Changes Of Serum And Urine Metabolites

Intermediary metabolism, including renal metabolism, is increasingly recognized as an important contributor to the regulation of blood pressure and the development of hypertension, a leading risk factor for disease burden worldwide. Dietary salt intake has significant effects on blood pressure. We test the hypothesis that human blood pressure on low- or high-sodium intake is associated with divergent changes of serum and urinary metabolites. A gas chromatography and mass spectrometry analysis of 192 serum samples from the Dietary Approaches to Stop Hypertension 2 (DASH2 or DASH-Sodium) trial detected 384 metabolites and identified 116. Serum levels of citraconic acid, 1-monostearin and serotonin were associated with systolic or diastolic blood pressure on low- or high-sodium intake after adjustment for covariates (FDR < 0.05). Serum levels of 2-ketoisocaproic acid, glyceric acid, 1-monostearin, ornithine, and β-aminoisobutyric acid were associated with blood pressure differences between low- and high-sodium intakes (FDR < 0.05). An integrated analysis of 33 amino compounds and tricarboxylic acid cycle intermediates indicated that serum and urinary levels were not significantly correlated (FDR < 0.05) for the majority of these metabolites. Blood pressure appeared to be associated with serum, but not urinary, levels of leucine and cystine and urinary, but not serum, levels of β-aminoisobutyric acid. These novel findings support a role for several serum metabolites and renal handling of metabolites in the regulation of human blood pressure on low- or high-sodium intake.