Method Development and Validation for Simultaneous Determination of Levofloxacin and Diclofenac Sodium in Pharmaceutical Dosage Form

A simple, cost effective specific and accurate reverse phase liquid chromatographic method has been developed and validated for the simultaneous determination of levofloxacin and diclofenac sodium. The method was validated in terms of linearity, precision, accuracy, and specificity, limit of detection and limit of quantitation. The separation was carried out using Waters Spherisorb 5 mu m ODSI 4.6 x 250 mm analytical column with isocratic mode. The mobile phase was comprised of methanol and water (80: 20) with pH of 2.5 adjusted by ortho- phosphoric acid. The flow rate was maintained at 1mL/min and samples were analyzed at 230 nm. Mean retention time for levofloxacin and diclofenac sodium was found to be 1.912 and 6.940 minute respectively. The linearity for levofloxacin and diclofenac sodium was ranged between 0.195 mu g/mL to 25 mu g/mL. The percent potency of levofloxacin and diclofenac sodium was 99.733 and 99.066 correspondingly. The simplicity and short run time made the method more convenient for quantitative analysis and simultaneous determination of levofloxacin and diclofenac sodium.