Multi-wavelength Raman microspectroscopic studies of modified monwoven carbon scaffolds for tissue engineering applications

The articular cartilage is almost a non-vascular tissue, therefore it has a very low ability to heal itself. The analyzed carbon nonwoven material, obtained by polyacrylonitrile precursor carbonization, seem to be a new option for the complementation of cartilage losses due to a unique ability of this material to restore of cartilage tissue. The analyzed carbon material may be a new option to supplement cartilage defects due to the ability of this material to restore cartilage tissue. This study presents several examples of surface modification of nonwoven carbon fibers materials forming scaffold using ceramic nanoparticles, nanosilica and nanohydroksyapatite. Surface structural changes were examined by SEM observations, composition was assessed by EDS. The results indicate that the apatite phase on the surface of nonwoven fibers is formed, and its composition depends on the type of surface. The obtained multiwavelength Raman microspectroscopy results, G-band dispersion course and also the ID/IG intensity ratio dispersion, allowed to indicate the nanocrystaline graphite organization of the fibers with the presence of certain disorder. In this way, the resulting ordering in the tested materials also indicates the material’s production parameters. All scaffolds were evaluated by examining the ability of apatite to form on the materials incubated in simulated body fluids (SBF).