Lensless, reflection-based dark-field microscopy (RDFM) on a CMOS chip.

We present for the first time a lens-free, oblique illumination imaging platform for on-sensor dark- field microscopy and shadow-based 3D object measurements. It consists of an LED point source that illuminates a 5-megapixel, 1.4 mu m pixel size, back-illuminated CMOS sensor at angles between 0 degrees and 90 degrees. Analytes (polystyrene beads, microorganisms, and cells) were placed and imaged directly onto the sensor. The spatial resolution of this imaging system is limited by the pixel size (similar to 1.4 mu m) over the whole area of the sensor (3.6x2.73 mm). We demonstrated two imaging modalities: (i) shadow imaging for estimation of 3D object dimensions (on polystyrene beads and microorganisms) when the illumination angle is between 0 degrees and 85 degrees, and (ii) dark-field imaging, at >85 degrees illumination angles. In dark-field mode, a 3-4 times drop in background intensity and contrast reversal similar to traditional dark-field imaging was observed, due to larger reflection intensities at those angles. With this modality, we were able to detect and analyze morphological features of bacteria and single-celled algae clusters. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement