Antioxidant activities of various fruit extracts fr om three solanum sp . using DPPH and ABTS method and correlation with phenolic , flavonoid and carotenoid content

The objectives of this research were to study antio xidant activities from various fruit extracts of th ree Solanum sp using two methods of antioxidant assays which were DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2,2’ azinobis (3-ethyl-benzothiazoline-6-sulfonic acid) and correlation of total flavonoid, phenolic, and c arotenoid content in various fruit extracts of three Solanum sp with IC50 of DPPH antioxidant activities and IC 50 of ABTS antioxidant activities. Extraction was conducted by reflux apparatus using different polarity solvents . The extracts were evaporated using rotary evaporator. Antioxidan t capacities were tested using DPPH and ABTS assays . Determination of total phenolic, and carotenoid con tent performed by UV-visible and their correlation with IC50 of DPPH and IC50 of ABTS antioxidant activities were analyzed by Pe arson’s method. Ethanolic fruit extract of S. torvum (ST3) had the lowest IC 50 of DPPH scavenging activity 1.4 μg/ml, while ethan olic fruit extract of S. coagulans (SC3) had the lowest IC 50 of ABTS scavenging activity 1.5 μg/ml. Ethanolic f ruit extract of S. torvum (ST3) the highest total phenolic content (5.15 g GA E/100 g), ethyl acetate fruit extract of S. america num (SA2) had the highest total flavonoid content (9.37 g QE/100 g), and the highest total carotenoid content (0.87 g BE/100 g). There were negatively high correlation between tota l phenolic content in all of fruit extracts of S. a mericanum, S. torvum, S. coagulans with their IC 50 of DPPH and IC50 of IC50 of ABTS scavenging activities. The IC 50 of DPPH scavenging activities of S. americanum, S. torvum, S. coagulans fruit extracts gave linear result with their IC50 of ABTS scavenging activities.