Production of Cysteine Proteases by Recombinant Microorganisms: A Critical Review

Cysteine Proteases are groups of enzymes which able to degrade protein. These enzymes are commonly found in nature in many plant families and rich in known fruits such as in papaya, pineapple, and kiwi. However, the highest enzyme concentration is usually obtained from unripe fruits. In addition to their traditional uses of cysteine proteases as meat tenderizers, they have nowadays wider applications in the field of food, pharma, and textile industries. Beside the traditional extraction of these enzymes from tropical fruits, especially from papaya fruit, this enzyme was successfully expressed in many microbial cells for industrial production of this important group of enzymes. This review is focused on providing more updated information about the recent development in the production of cysteine proteases using recombinant microorganisms belong to different classes of biofactories.