Isolation of Omega 3 Fatty Acid from Fish Oil

Omega 3 fatty acids containing Eicosapentaenoic acid (EPA) and Docosahexaenoic acid (DHA) play a beneficial role in human health and as functional food. In this study fish oil was purchased from Fish oil and Fish meal extraction factory, Mangalore. The standards were purchased from sigma Aldrich. The fish oil was further characterized by various analytical techniques such as High performance Liquid chromatography (HPLC), thin layer chromatography (TLC), Fourier infrared spectroscopy (FTIR) in order to isolate omega 3 fatty acids from fish oil. Quality of fish oil was analysed in order to determine impurities, acid value and peroxide value of fish oil which was in recommended range. Traditional extraction of omega 3 fatty acids are expensive and time consuming. Basically high yield and concentrated omega 3 fatty acids from sardine fish oil were obtained by supercritical fluid extraction, urea complexation, low temperature crystallization and molecular distillation, enzymatic hydrolysis. The main aim to enhance and improve the concentration of polyunsaturated omega 3 fatty acids from saradine fish oil and production of biodiesel through Tansesterification after recovery of omega 3 fatty acids.