The role of calcium utilizatio n of intestinal flora on urinary calcium excretio n 1f

We aimed to evaluate whether Ca+2 utilization of intestinal flora (IF) has an effect on urinary excretion o f Ca+2 (UECa) levels. 0.1g/ml feces samples of children who underwent UECa examination in the last year were implanted in broths. 5 pL of labeled 45Ca was added to the samples and incubated. 200 pL of the samples were filtrated by 0.45 micrometer membrane and rinsed by 200 pL pure water. 45Ca activity in the membrane was counted and defined as percent activity per bacteria (45Caact %/CFU). Levels of aerobic and anaerobic 45CaaCt %/CFU and their correlations with UECa were compared between hypercalciuric (Group I) and normocalciuric (Group II) patients. 45Caact %/CFU levels were similar between the groups (p>0.05). UECa were negatively but not significantly correlated to aerobic and anaerobic 45CaaCt%/CFU (p:0,079, r:0. 503. p:0.260, r:-0.420, respectively) in hypercalciuric patients. In normocalciuric patients, levels were correlated positively to aerobic and negatively to anaerobic 45Caact%/CFU again in an insignificant manner (p:0.509, r: 0.223; p:0.623, r:-0.257, respectively). Similar 45Caact%/CFU levels in both groups imply that calcium utilization of IF does not have a distinct effect on UECa but, although not significant, there is a negative correlation between UECa and bacterial 45Ca/CFU levels in hypercalciuric patients.