Transcriptional Activity Of Vitamin D Receptor In Human Periodontal Ligament Cells Is Diminished Under Inflammatory Conditions

Background Although vitamin D(3)deficiency is considered as a risk factor for periodontitis, supplementation during periodontal treatment has not been shown to be beneficial to date. Human periodontal ligament cells (hPDLCs) are regulated by vitamin D(3)and play a fundamental role in periodontal tissue homeostasis and inflammatory response in periodontitis. The aim of this study is to investigate possible alterations of the vitamin D(3)activity in hPDLCs under inflammatory conditions. Methods Cells isolated from six different donors were treated with either 1,25(OH)(2)D-3(0 to 10 nM) or 25(OH)D-3(0 to 100 nM) in the presence and absence of ultrapure or standardPorphyromonas gingivalislipopolysaccharide (PgLPS), Pam3CSK4, or interferon-gamma for 48 hours. Additionally, nuclear factor (NF)-kappa B inhibition was performed with BAY 11-7082. The bioactivity of vitamin D in hPDLCs was assessed based on the gene expression levels of vitamin D receptor (VDR)-regulated genes osteocalcin and osteopontin. Additionally, VDR and CYP27B1 expression levels were measured. Results The vitamin D-3-induced increase of osteocalcin and osteopontin expression was significantly decreased in the presence of standardPgLPS and Pam3CSK4, which was not observed by ultrapurePgLPS. Interferon-y had diverse effects on the response of hPDLCs to vitamin D(3)metabolites. NF-kB inhibition abolished the effects of standardPgLPS and Pam3CSK4. StandardPgLPS and Pam3CSK4 increased VDR expression in the presence of vitamin D-3. CYP27B1 expression was not affected by vitamin D(3)and inflammatory conditions. Conclusions This study indicates that the transcriptional activity of VDR is diminished under inflammatory conditions, which might mitigate the effectiveness of vitamin D(3)supplementation during periodontal treatment.