A Novel Role Of Lrp5 In Tubulointerstitial Fibrosis Through Activating Tgf-Beta/Smad Signaling

Previous studies by us and others demonstrated that activation of Wnt/beta -catenin signaling plays a pathogenic role in chronic kidney diseases (CKD). Wnt co-receptor LRP5 variants are reported to associate with autosomal dominant polycystic kidney disease; but their exact roles in this disease and renal fibrosis have not been explored. Here, we observed the upregulation of LRP5 in the renal tubules of both type 1 and type 2 diabetic models and of an obstructive nephropathy model. In the obstructed kidneys, Lrp5 knockout significantly ameliorated tubulointerstitial fibrosis and tubular injury without changing Wnt/beta -catenin signaling. Instead, decreased levels of TGF-beta 1 and TGF-beta receptors (T beta Rs) were detected in Lrp5 knockout kidneys, followed by attenuated activation and nuclear translocation of Smad2/3 in the renal tubules, suggesting a regulatory effect of LRP5 on TGF-beta /Smad signaling. In consistent with this hypothesis, LRP5 overexpression resulted in enhanced TGF-beta /Smad signaling activation in renal tubule epithelial cells. Furthermore, LRP5 was co-immunoprecipitated with T beta RI and T beta RII, and its extracellular domain was essential for interacting with T beta Rs and for its pro-fibrotic activity. In addition to stabilizing T beta Rs, LRP5 increased the basal membrane presentation and TGF-beta 1-induced internalization of these receptors. Notably, TGF-beta 1 also induced LRP5 internalization. These findings indicate that LRP5 promotes tubulointerstitial fibrosis, at least partially, via direct modulation of TGF-beta /Smad signaling, a novel, Wnt-independent function.