Protection by collagen peptides from walleye pollock skin on bone formation via inhibition of oxidative stress

Abstract Background Collagen peptides (CP) have been shown to protect against osteoporosis. Yet, its mechanism on anti-osteoporosis remains unclear. Method Cell viability, levels of alkaline phosphatase (ALP), reactive oxygen species (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) of hydrogen peroxide (H2O2)-induced oxidative stress in Saos-2 cells pretreated with CP were examined. The effect and regulatory of CP on FBXW7-p100-NF-κB axis and the relationship between the molecules were explored by overexpressing FBXW7, silencing p100 or NF-κB in Saos2 cells. Western blot and RT-PCR were performed to determine the expressions of FBXW7, p100 and NF-κB. Hematoxylin u0026 Eosin (Hu0026E) staining was performed to determine morphological indexes of bone tissues in ovariectomized-induced osteoporosis rats treated with CP. Result CP increased cell viability, ALP and antioxidant activities in H2O2-induced oxidative stress in Saos-2 cells. Results on cell viability, ALP, antioxidant levels, protein and gene expression levels in FBXW7-overexpressed, p100-silenced and NF-κB-silenced Saos-2 cells indicate that FBXW7, p100 and NF-κB are the key regulators and CP treatment modulates FBXW7-p100-NF-κB axis in Saos2 cells. In addition, pretreatment and treatment with CP in ovariectomized rats improved osteoporosis. Conclusion CP could effectively prevent and alleviate osteoporosis. The protective effect of CP on osteoporosis was achieved in part by inhibiting oxidative damage of osteoblasts and promoting bone formation.