Presence of Vibrio mediterranei associated to major mortality in stabled individuals of Pinna nobilis L.

Event Abstract Back to Event Presence of Vibrio mediterranei associated to major mortality in stabled individuals of Pinna nobilis L. PATRICIA PRADO1*, GAETANO CATANESE2, Amalia G. Jofre2, Karl B. Andree1, Jose Rafael Garcia March3, Pep Cabanes1, Francesca Carella4, Jose Tena3 and Noèlia Carrasco1 1 Institute of Agrifood Research and Technology (IRTA), Spain 2 Marine Research And Aquaculture Laboratory (LIMIA), Spain 3 Catholic University of Valencia San Vicente Mártir, Spain 4 University of Naples Federico II, Italy A large number of works have established bacterial implications in bivalve disease outbreaks within maintenance tanks. Usual stabling conditions linked to animal density, high temperatures and addition of food, can favor the growth of high concentrations of bacteria belonging to Vibrio sp. A rescue project of 221 adult individuals of the endangered pen shell, Pinna nobilis L. was conducted in November 2017 in two areas of the Spanish coast where the species was still abundant and apparently free from the parasitic disease caused by Haplosporidium pinnae: Port Lligat in the Costa Brava, and the Alfacs Bay in the Ebro Delta. For biosecurity reasons, the 106 individuals from the Ebro Delta were stabled at the IRTA facilities located next to the Alfacs Bay, whereas the 115 individuals from Port Lligat were stabled in different institutions throughout the Spanish territory. Initial biopsies showed that individuals from the Ebro Delta were free of the parasite whereas most individuals from Port Lligat already were already parasitized and died in the following months. Individuals at IRTA were stabled in five tanks and fed ca. 4% of their DW with a mix of three species of phytoplankton and fine riverine sediments rich in OM (13%). Seawater was filtered through 1, 5 and 10 µm to ensure the absence of the parasite, and then disinfected and kept free of bacteria with UV light. No losses occurred during the 4 initial stabling months, but individuals started to die in April-May 2018 at temperatures from 16 to 19 C. A peak of mortality occurred during the summer months and early fall (53%) of with maximums coinciding with temperatures above 25 C and decreased during the winter period. Individuals were analyzed with PCR and histology for the presence of H. pinnae, Mycobacteria sp., Vibrio splendidus, V. aestuarianus and Herpesvirus microvar, considered important pathogens of pen shells or commercial bivalves also present in the Alfacs Bay, but results were all negative. Microbiological culture and isolation of bacteria from three ailing individuals sacrificed for study purposes demonstrated V. mediterranei as the dominant species, and further PCR analyses in deceased individuals also identified the presence of the bacteria. Overall, our results suggest that V. mediterranei is an important opportunistic pathogen of stabled individuals possibly subjected to captivity stress, and that antibiotic treatment (Florfenicol) combined with vitamins and controlled water temperature (18ºC) can be used to control the disease. Further research is needed to develop specific PCR and qPCR techniques that could allow a more adequate follow-up and pathogenesis of this emerging disease. Figure 1 Image 1 Acknowledgements Authors are grateful to the Spanish government (MITECO) for financial support and to the Servei de Fauna i Flora (Generalitat de Catalunya) and the Ebro Delta Natural Park for granting the necessary permits. Keywords: Captivity stress, pen shell disease, antibiotic treatment, temperature, Vitamins Conference: XX Iberian Symposium on Marine Biology Studies (SIEBM XX) , Braga, Portugal, 9 Sep - 12 Sep, 2019. Presentation Type: Oral Presentation Topic: Global Change, Invasive Species and Conservation Citation: PRADO P, CATANESE G, Jofre AG, Andree KB, Garcia March J, Cabanes P, Carella F, Tena J and Carrasco N (2019). Presence of Vibrio mediterranei associated to major mortality in stabled individuals of Pinna nobilis L.. Front. Mar. Sci. Conference Abstract: XX Iberian Symposium on Marine Biology Studies (SIEBM XX) . doi: 10.3389/conf.fmars.2019.08.00142 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Jun 2019; Published Online: 27 Sep 2019. * Correspondence: Dr. PATRICIA PRADO, Institute of Agrifood Research and Technology (IRTA), Cabrils, Spain, patricia.prado@irta.cat Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers PATRICIA PRADO GAETANO CATANESE Amalia G Jofre Karl B Andree Jose Rafael Garcia March Pep Cabanes Francesca Carella Jose Tena Noèlia Carrasco Google PATRICIA PRADO GAETANO CATANESE Amalia G Jofre Karl B Andree Jose Rafael Garcia March Pep Cabanes Francesca Carella Jose Tena Noèlia Carrasco Google Scholar PATRICIA PRADO GAETANO CATANESE Amalia G Jofre Karl B Andree Jose Rafael Garcia March Pep Cabanes Francesca Carella Jose Tena Noèlia Carrasco PubMed PATRICIA PRADO GAETANO CATANESE Amalia G Jofre Karl B Andree Jose Rafael Garcia March Pep Cabanes Francesca Carella Jose Tena Noèlia Carrasco Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.